<tt id="2urzx"></tt>

<tt id="2urzx"><ol id="2urzx"></ol></tt><listing id="2urzx"><strong id="2urzx"><s id="2urzx"></s></strong></listing><label id="2urzx"></label>
<label id="2urzx"><ruby id="2urzx"></ruby></label>

    1. <output id="2urzx"><ruby id="2urzx"></ruby></output>

      1. <label id="2urzx"><sup id="2urzx"></sup></label>
      2. 细胞鉴定简介:

        近年来,大量研究表明 STR 基因分型 方法是进行细胞交叉污染和性质鉴定的最有效和准确的方法之一,STR基因分型应用于细胞鉴定已被ATCC等机构强烈推荐。美国的ATCC 细胞库、德国的DSMZ细胞库以及日本的JCRB细胞库等为STR 分型提供了各细胞株的数据供比对。

        STR基因位点由长度为27个碱基对的短串连重复序列组成,这些重复序列广泛存在于人类基因组中,可作为高度多态性标记,被称为细胞的DNA指纹,其可通过PCR(聚合酶链式反应)来检测。STR基因座位上的等位基因可通过扩增区域内重复序列的拷贝数的不同来区分,在毛细管电泳分离之后可通过荧光检测来识别。随后通过一定的计算方法,即可根据所得的STR分型结果与专业的细胞STR数据库比对从而推算出样品所属的细胞系或可能的交叉污染的细胞系名称。


        实验方案:

        样本要求:

        细胞团>10^6个,或一瓶活细胞,或冻存细胞>10^6个;或DNA浓度>50ng/μl,体积>20μl


        有细胞想鉴定?快戳这里……

        参考文献:

        1.  Chatterjee, R. (2007) Cell biology. Cases of mistaken identity. Science 315, 928-31.

        2.   Ruiz Bravo, N. and Gottesman, M. (2007) Notice regarding authentication of cultured cell lines. This can be viewed online at:

        http://grants.nih.gov/grants/guide/notice-files/NOT-OD-08-017.html

        3.   Yoshino, K. et al. (2006) Essential role for gene profiling analysis in the authentication of human cell lines. Human Cell 19, 43–8.

        4.   Szibor, R. et al. (2003) Cell line DNA typing in forensic genetics—the necessity of reliable standards. Forensic Sci. Int. 138, 37–43.

        5.   Dirks, W.G. et al. (2005) Short tandem repeat DNA typing provides an international reference standard for authentication of human cell lines. ALTEX 22, 103–9.

        6.   Masters, J.R. et al. (2001) Short tandem repeat profiling provides an international reference standard for human cell lines. Proc. Natl. Acad. Sci. USA 98, 8012–7.

        7.   (2001) Verify cell line identity with DNA profiling. ATCC Connection: Newsletter of The American Type Culture Collection 21, 1–2.

        8.   Krenke, B. et al. (2002) Validation of a 16-locus fluorescent multiplex system. J. Forensic Sci. 47, 773–85.

        9.   Levinson, G. and Gutman, G.A. (1987) Slipped-strand mispairing: A major mechanism for DNA sequence evolution. Mol. Biol. Evol. 4, 203–21.

        10. Schlotterer, C. and Tautz, D. (1992) Slippage synthesis of simple sequence DNA. Nucleic Acids Res. 20, 211–5.

        11. Smith, J.R. et al. (1995) Approach to genotyping errors caused by nontemplated nucleotide addition by Taq DNA polymerase. Genome Res. 5, 312–7.

        12. Magnuson, V.L. et al. (1996) Substrate nucleotide-determined non-templated addition of adenine by Taq DNA polymerase: Implications for PCR-based genotyping. BioTechniques 21, 700–9.

        13. Walsh, P.S., Fildes, N.J. and Reynolds, R. (1996) Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA. Nucleic Acids Res. 24, 2807–12.

        14. Moller, A., Meyer, E. and Brinkmann, B. (1994) Different types of structural variation in STRs: HumFES/FPS, HumVWA and HumD21S11. Int. J. Leg. Med. 106, 319–23.

        15. Brinkmann, B., Moller A. and Wiegand, P. (1995) Structure of new mutations in 2 STR systems. Int. J. Leg. Med. 107, 201–3.

        16. Griffiths, R. et al. (1998) New reference allelic ladders to improve allelic designation in a multiplex STR system. Int. J. Legal Med. 111, 267–72.

        17. B?r, W. et al. (1997) DNA recommendations: Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. Int. J. Legal Med. 110, 175–6.

        18. Gill, P. et al. (1997) Considerations from the European DNA Profiling Group (EDNAP) concerning STR nomenclature. Forensic Sci. Int. 87, 185–92.

        亚洲mm8成为人电影网 超碰国产天天做天天爽| 大乳女做爰中文字幕| 哥哥干狠狠日| 野外啪啪视频| 国际中文字幕无线乱码| 看欧美av片| 2017天天日天天干天天操| 久久草影院在线视频观看| 午夜福利不卡片在线播放| 久久草首页| 黄瓜啪啪视频| 伊人天天干天天插| 久久一日本道色综合久久| 狠狠插狠狠日狠狠干| 久久爱久草视频| 婷婷综合开心五月| 天天射天天日天天干2017| 亚洲色丁香五月| 最新99久久网址官网| 天天啪天天干| 大香蕉伊人色在线视频| 色婷婷开心五月综合| 香蕉伊人综合在线| 香蕉依伊人久久网| 午夜福利国产2000| 成人五月丁香| 天天干天天天鲁天天射| 久久草免费| 开心网五月天婷婷丁香| 丁香亚洲五月| 伊人综合在线视频在线视频| 狠狠地搞狠狠地干| 日本一级色片| 色男人的天堂| 久久综合九色综合欧美| 婷婷色香五月综合激情| 五月婷婷和香六月丁香| 大香蕉伊人日本| 免费av欧美国产在钱| 中文字幕久荜在线| 99热大香蕉伊人|